Genotyping and primer details


Genotyping and primer details for SALK_003454c - At1g29640
Line ID SALK_003454c
Locus At1g29640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10356041 of line SALK_003454c Chr1:10356041
   
Insertion allele amplicon LBb3 - 17ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17ux: 5'-CATCATCACATAACCACACACAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10356577
   
wt amplicon (over InsPos) 55vl - 17ux
wt amplicon start Chr1:10355507
wt amplicon end Chr1:10356577
wt amplicon size 1071 bp
Locus specific primer 17ux: 5'-CATCATCACATAACCACACACAAA-3'
2nd locus specific primer 55vl: 5'-CCAGAAATGTCTCTTTCTTGCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11