Genotyping and primer details


Genotyping and primer details for SALK_003739 - At2g07750
Line ID SALK_003739
Locus At2g07750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:3576951 of line SALK_003739 Chr2:3576951
   
Insertion allele amplicon LBb3 - 33wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33wm: 5'-ATTACTCATTTTCGCATCAAATCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:3576348
   
wt amplicon (over InsPos) 31xa - 33wm
wt amplicon start Chr2:3576348
wt amplicon end Chr2:3577638
wt amplicon size 1291 bp
Locus specific primer 33wm: 5'-ATTACTCATTTTCGCATCAAATCG-3'
2nd locus specific primer 31xa: 5'-ATCGTTATCGTTCCACAAATCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11