Genotyping and primer details


Genotyping and primer details for SALK_004298c - At5g47770
Line ID SALK_004298c
Locus At5g47770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19345797 of line SALK_004298c Chr5:19345797
   
Insertion allele amplicon LBb3 - 12ck
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12ck: 5'-TTGAAGTTACTGGAGGAGAGAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19344945
   
wt amplicon (over InsPos) 22dn - 12ck
wt amplicon start Chr5:19344945
wt amplicon end Chr5:19345805
wt amplicon size 861 bp
Locus specific primer 12ck: 5'-TTGAAGTTACTGGAGGAGAGAAGC-3'
2nd locus specific primer 22dn: 5'-AAACCTCTTGCTCAGTCAAATCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11