Genotyping and primer details


Genotyping and primer details for SALK_004733c - At4g24190
Line ID SALK_004733c
Locus At4g24190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12554825 of line SALK_004733c Chr4:12554825
   
Insertion allele amplicon LBb3 - 11jg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 11jg: 5'-AGTGTCTGTGTTGGTGATTTTCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12555259
   
wt amplicon (over InsPos) 04kp - 11jg
wt amplicon start Chr4:12554323
wt amplicon end Chr4:12555259
wt amplicon size 937 bp
Locus specific primer 11jg: 5'-AGTGTCTGTGTTGGTGATTTTCAG-3'
2nd locus specific primer 04kp: 5'-AGAGTTTGGGGTTCTCTACAACAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11