Genotyping and primer details

Genotyping and primer details for SALK_005087c - At1g02820
Line ID SALK_005087c
Locus At1g02820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:624256 of line SALK_005087c Chr1:624256
Insertion allele amplicon LBb3 - 51cs
Locus specific primer 51cs: 5'-TTTGTGATTCTACCGGTTTTTACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:623500
wt amplicon (over InsPos) 83dy - 51cs
wt amplicon start Chr1:623500
wt amplicon end Chr1:624487
wt amplicon size 988 bp
Locus specific primer 51cs: 5'-TTTGTGATTCTACCGGTTTTTACC-3'
2nd locus specific primer 83dy: 5'-TCTCTTCCTTCTCATCTATCCTGC-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11