Sequences from confirmation amplicon


Confirmation sequences for line SALK_005087c and hit At1g02820 are shown.
Line ID SALK_005087c
Locus At1g02820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:624256
Primers used for PCR 51cs - LBb3, show primer details
   
Sequence Name 83-K109033-SALK_005087C-51cs
GenBank Accession LN486256 [GenBank]
Sequencing primer gene specific primer, 51cs: 5'-TTTGTGATTCTACCGGTTTTTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K109033-SALK_005087C-51cs
TATTATTTTTGGTAGGAGCATAGTCTATATTATTGTATGTATGTTACAATACTAGAATTT
ATGCTCTTTTTATACAAAGAATAAAGGCCCTGTAATGTTTTTTCTTTGTAGTTTCGTCAA
AATAATATTTATACCAATTGAATTTTTTTAAAGTATTTTTTTTGTCATCACTTTGAGTCA
TAGCAAATCTTTGTTAATGGGAAGATGGAAGCCTTTAAAACTTCATAAATAATAAGATCA
AAATAATAAGATCAAACGATTATTACAGAAATGGTAACGGAATAAGATGGGATTCACCAC
AAAAGATCATAATAACCCATTTTGCTAATCTCCTCATTAAACAACATTGATGAATAAAAA
TACGGTTTTTAATAAATTTATTGCTTGTTGTTCAAGAGAATAGCTCGTAGCTCAGCCGGG
TCAATCTCCTCAGAAACGGTTTCCGGTCTGTAATAACCGGTTTTAGGATCTGGAACCCAT
GGTGCCTTCTCGCTTGAAGCTTCCCCAGCTCTCTTCTTCATCTCCGCGGGCGAAACGCTT
CCATCTAACGCCGTTTTGGTTGCGGCGGC
   
Sequence Name 67-K109032-SALK_005087C-R210
GenBank Accession LN485921 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K109032-SALK_005087C-R210
CGTCATTTGTTTACCACAATATATTCAGTTGGGTGAAAAGCTCTCGAACGCCGTCTTCAG
GTAACATCTGTTTCTGAAACAAAAACGTTATGTATTGCTCTGTTTTCTCGTCGCTTTACA
AAACGCTGACGTTTTGGATTTTGGATTCTCTTCAGACGAGGGTTTGCCGCCGCAGCCAAA
ACGGCGTTAGATGGAAGCGTTTCGACCGCGGAGATGAAGAAGAGAGCTGGGGAAGCTTCA
AGCGAGAAGGCACCATGGGTTCCAGATCCTAAAACCGGTTATTACAGACCGGAAACCGTT
TCTGAGGAGATTGACCCGGCTGAGCTACGAGCTATTCTCTTGAACAACAAGCAATAAATT
TATTAAAAACCGTATTTTTATTCATCAATGTTGTTTAATGAGGAGATTAGCAAAATGGGT
TATTATGATCTTTTGTGGTGAATCCCATCTTATTCCGTTACCATTTCTGTAATAATCGTT
TGATCTTATTATTTTGATCTTATTATTTATGAAGTTTTAAAGGCTTCCATCTTCCCATTA
ACAAAGATTTGCTATGACTCAAAGTGATGACCAAAAAAATACTTTAAAAAAATTCAATTG
GTATAAATATTATTTTGACGAAACTACAAAGAAAAAACATTGCATGGCCTTTATTCTTTG
TATAAAAAGAGCATAAAATTGGAGTTACTTAACATACATACCATAATATAGACTTAATCT
CCTTACC

Last Updated on 06.12.2018 16:11