Genotyping and primer details


Genotyping and primer details for SALK_005571c - At5g48590
Line ID SALK_005571c
Locus At5g48590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19699620 of line SALK_005571c Chr5:19699620
   
Insertion allele amplicon LBb3 - 17og
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17og: 5'-TTGGTTATCACTATGGAATGGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19698985
   
wt amplicon (over InsPos) 02om - 17og
wt amplicon start Chr5:19698985
wt amplicon end Chr5:19700057
wt amplicon size 1073 bp
Locus specific primer 17og: 5'-TTGGTTATCACTATGGAATGGTTG-3'
2nd locus specific primer 02om: 5'-TTGATGAGAGATGAGAAACTGGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11