Sequences from confirmation amplicon


Confirmation sequences for line SALK_005571c and hit At5g48590 are shown.
Line ID SALK_005571c
Locus At5g48590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19699620
Primers used for PCR 17og - LBb3, show primer details
   
Sequence Name 48-K114681-SALK_005571C-17og
GenBank Accession LN485458 [GenBank]
Sequencing primer gene specific primer, 17og: 5'-TTGGTTATCACTATGGAATGGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K114681-SALK_005571C-17og
CTTTATTGGAGACTGATAACGTATTTGAATCGATGTTTTGCAGAAGGATAGCTCAACTTA
AGGAGAGTGATTGCCAAATTGCAATTGAAGACATAATGTACATGTTGATCTTGTACAAAT
TCTCAGAGATAAGAGTGCCTCTTGTTCCAAAGCTTCCCAGTTGCATTTACAACGGAAGAC
TCGAGATCTCGCCTTCGAAAGACTGGGAACTTGAGTCAATCCATAGCTTTGACGTTCTTG
AACTCATCAAAGAACATAGTAACGCAGTCATCAGTTTAAGAGTGAATTCGAGTTTGACTG
ACGATTGTGCGACAACGGAGATAGATAAGAATCGTCTCAGTAAAGTATACACAGCTTCGG
TCTTATATGGATACTTTTTAAAATCTGCTTCTCTTAGACACCAACTCGAATGCTCATTAT
CACAACACCATGGAAGTTTCACTAAGCAACTGAGGCATTACATTTCTGAGTTCGATCCCA
AGATATTGCGGAGATGCGCAAAACCAAGGTCACATGAAGCAAAAAGCCTGATAGAAAAGC
AGAGTTTAGCTCTTTTTGGTCCTGAGGAAAGCAGCAAAGAGAGCATTGTGACATCGTTTT
CTAGACAAATTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTC
ACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGC
AAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 24-K114680-SALK_005571C-R210
GenBank Accession LN484980 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K114680-SALK_005571C-R210
CTCTCTTTGCTGCTTTCCTCGGACCAAAAAGAGCTAAACTCTGCTTTTCTATCAGGCTTT
TTGCTTCATGTGACCTTGGTTTTGCGCATCTCCGCAATATCTTGGGATCGAACTCAGAAA
TGTAATGCCTCAGTTGCTTAGTGAAACTTCCATGGTGTTGTGATAATGAGCATTCGAGTT
GGTGTCTAAGAGAAGCAGATTTTAAAAAGTATCCATATAAGACCGAAGCTGTGTATACTT
TACTGAGACGATTCTTATCTATCTCCGTTGTCGCACAATCGTCAGTCAAACTCGAATTCA
CTCTTAAACTGATGACTGCGTTACTATGTTCTTTGATGAGTTCAAGAACGTCAAAGCTAT
GGATTGACTCAAGTTCCCAGTCTTTCGAAGGCGAGATCTCGAGTCTTCCGTTGTAAATGC
AACTGGGAAGCTTTGGAACAAGAGGCACTCTTATCTCTGAGAATTTGTACAAGATCAACA
TGTACATTATGTCTTCAATTGCAATTTGGCAATCACTCTCCTTAAGTTGAGCTATCCTTC
TGCAAAACATCGATTCAAATACGTTAATCAGTCTCCATATAAAAGCCAGATCATAGAACA
TAAAACAGAAACAACCATTCCA

Last Updated on 06.12.2018 16:11