Genotyping and primer details


Genotyping and primer details for SALK_005849c - At5g26740
Line ID SALK_005849c
Locus At5g26740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9292946 of line SALK_005849c Chr5:9292946
   
Insertion allele amplicon LBb3 - 16ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16ea: 5'-TTCCCTTTAGCATAAAGCACAAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9293420
   
wt amplicon (over InsPos) 08fi - 16ea
wt amplicon start Chr5:9292743
wt amplicon end Chr5:9293420
wt amplicon size 678 bp
Locus specific primer 16ea: 5'-TTCCCTTTAGCATAAAGCACAAGT-3'
2nd locus specific primer 08fi: 5'-TTGATTCCATACGAGAAGTGTGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11