Genotyping and primer details


Genotyping and primer details for SALK_006148c - At3g10090
Line ID SALK_006148c
Locus At3g10090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3109597 of line SALK_006148c Chr3:3109597
   
Insertion allele amplicon LBb3 - 17ay
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17ay: 5'-GAAACCAGTTAATTATTGGTGCGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3110032
   
wt amplicon (over InsPos) 01cd - 17ay
wt amplicon start Chr3:3109143
wt amplicon end Chr3:3110032
wt amplicon size 890 bp
Locus specific primer 17ay: 5'-GAAACCAGTTAATTATTGGTGCGT-3'
2nd locus specific primer 01cd: 5'-CTGAGAATCCATTCTGATTCAACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11