Genotyping and primer details


Genotyping and primer details for SALK_006503c - At1g18260
Line ID SALK_006503c
Locus At1g18260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6279083 of line SALK_006503c Chr1:6279083
   
Insertion allele amplicon LBb3 - 12jg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12jg: 5'-GCTTATGGCTCCGTAGAAACTATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6279428
   
wt amplicon (over InsPos) 05km - 12jg
wt amplicon start Chr1:6278753
wt amplicon end Chr1:6279428
wt amplicon size 676 bp
Locus specific primer 12jg: 5'-GCTTATGGCTCCGTAGAAACTATG-3'
2nd locus specific primer 05km: 5'-CAAGTTATGTTTGTCGTTGCACTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11