Sequences from confirmation amplicon


Confirmation sequences for line SALK_006503c and hit At1g18260 are shown.
Line ID SALK_006503c
Locus At1g18260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6279083
Primers used for PCR 12jg - LBb3, show primer details
   
Sequence Name 18-K113259-SALK_006503C-12jg
GenBank Accession LN484783 [GenBank]
Sequencing primer gene specific primer, 12jg: 5'-GCTTATGGCTCCGTAGAAACTATG-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K113259-SALK_006503C-12jg
ACCGTACATTTTCTCATTCTTATGTTAAATAGTTGGATGCAGTCTAACAGATCAAAATAA
CAAGAGGAAATTGTTTTTTTTTTACTGATAGGTGAGAGTTGTGGATTCATTACCAGAAGT
GTATCCAAAGGTAGAGACTTGGATAGAGAATGTGGTGTTTGAAGAAGGTAATGCAACAAT
ACTTACACTCTTTGTCTGTCTCATCACAATTCTCTATCTCCGGGAAAGACAACGTAAACA
AGTCGTCGTTGTTGCCGATCCAGTGGCTGCTGACGTGGCACAACCGCTCGACGCTCGACG
CTCGACGCTCGACGCTGACGTGGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGA
CAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAG
TGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCG
GTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCAAAATCGGC
AAA
   
Sequence Name 01-K113258w-SALK_006503C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K113258w-SALK_006503C-R210
ACCACAATATATCCTGCCACNTCAGCGTCGAGCGTCGAGCGTCGAGCGTCGAGCGGTTGT
GCCACGTCAGCAGCCACTGGATCGGCAACAACGACGACTTGTCTACGTTGTCTTTCCCGG
AGATAGAGAATTGTGATGAGACAGACAAAGAGTGTAAGTATTGTTGCATTACCTTCTTCA
AACACCACATTCTCTATCCAAGTCTCTACCTTCGGATACACTTCTGGTAATGAATCCACA
ACTCTCACCTATCAGTAAAAAAAAAACAATTTCCTCTTGTTATTTTGATCTGTTAGACTG
CATCCAACTATTTAACATAAGAATGAGAAAATGTACGGTTTTTAGATTTTACCAGGACGG
TATCAGCATAGTTTCTACGGAGCCATAA

Last Updated on 06.12.2018 16:11