Genotyping and primer details


Genotyping and primer details for SALK_006564c - At4g26120
Line ID SALK_006564c
Locus At4g26120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13237860 of line SALK_006564c Chr4:13237860
   
Insertion allele amplicon LBb3 - 02bt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 02bt: 5'-TGGTGGAGGTTCTTTATCTGTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13236944
   
wt amplicon (over InsPos) 13lt - 02bt
wt amplicon start Chr4:13236944
wt amplicon end Chr4:13238264
wt amplicon size 1321 bp
Locus specific primer 02bt: 5'-TGGTGGAGGTTCTTTATCTGTCTT-3'
2nd locus specific primer 13lt: 5'-CTAGCAAGATGATTCAAGTCCTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11