Genotyping and primer details


Genotyping and primer details for SALK_007134c - At1g71230
Line ID SALK_007134c
Locus At1g71230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26853061 of line SALK_007134c Chr1:26853061
   
Insertion allele amplicon LBb3 - 22di
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22di: 5'-GAATGATTTAGGCCATATCCAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26853590
   
wt amplicon (over InsPos) 06ej - 22di
wt amplicon start Chr1:26852518
wt amplicon end Chr1:26853590
wt amplicon size 1073 bp
Locus specific primer 22di: 5'-GAATGATTTAGGCCATATCCAAGA-3'
2nd locus specific primer 06ej: 5'-AAACCCTAGATTTCTGCAACTGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11