Genotyping and primer details


Genotyping and primer details for SALK_007294c - At5g02970
Line ID SALK_007294c
Locus At5g02970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:697342 of line SALK_007294c Chr5:697342
   
Insertion allele amplicon LBb3 - 23vy
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23vy: 5'-CTCCTCCATTGTAGATCACAAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:696758
   
wt amplicon (over InsPos) 13wy - 23vy
wt amplicon start Chr5:696758
wt amplicon end Chr5:697871
wt amplicon size 1114 bp
Locus specific primer 23vy: 5'-CTCCTCCATTGTAGATCACAAACA-3'
2nd locus specific primer 13wy: 5'-GCCTAGATTTCGGAAGCTTTTATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11