Genotyping and primer details


Genotyping and primer details for SALK_007997c - At2g39180
Line ID SALK_007997c
Locus At2g39180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16344239 of line SALK_007997c Chr2:16344239
   
Insertion allele amplicon LBb3 - 61kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 61kh: 5'-ATGGAGATCTTTCACAACTGGACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16344789
   
wt amplicon (over InsPos) 20ls - 61kh
wt amplicon start Chr2:16343828
wt amplicon end Chr2:16344789
wt amplicon size 962 bp
Locus specific primer 61kh: 5'-ATGGAGATCTTTCACAACTGGACT-3'
2nd locus specific primer 20ls: 5'-CCAAAGTTGCTCTTGGTTTTTAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11