Genotyping and primer details


Genotyping and primer details for SALK_008144 - At5g18040
Line ID SALK_008144
Locus At5g18040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5973729 of line SALK_008144 Chr5:5973729
   
Insertion allele amplicon LBb3 - 62kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 62kh: 5'-GTTCACTATCGAAAACTTGCATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5973300
   
wt amplicon (over InsPos) 16mc - 62kh
wt amplicon start Chr5:5973300
wt amplicon end Chr5:5974122
wt amplicon size 823 bp
Locus specific primer 62kh: 5'-GTTCACTATCGAAAACTTGCATCA-3'
2nd locus specific primer 16mc: 5'-TTGAGACCACAAACAGAGTTTCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11