Sequences from confirmation amplicon


Confirmation sequences for line SALK_008144 and hit At5g18040 are shown.
Line ID SALK_008144
Locus At5g18040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5973729
Primers used for PCR 62kh - LBb3, show primer details
   
Sequence Name 69-K113949-SALK_008144C-62kh
GenBank Accession LN485982 [GenBank]
Sequencing primer gene specific primer, 62kh: 5'-GTTCACTATCGAAAACTTGCATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K113949-SALK_008144C-62kh
TAGATGTCAGGGAGAGGGAGCAACCACTCACTGTCAAGACTGCCTTGAATGTGCTTTCAA
GAAAGGGCTACCGAGAAGAGAACATTGGGCACATGTGGGATGTACATTCAAGGCTCCCCC
ATTTGCTTGTCACATACCCCGCGTGCCCATGAAAGGAGAAGTGATTGAGACTAAGAGTTT
GGATGAAGCGTTTAAGCTGTTGATTAAACAACCGGTGGGTGCAAGACTCCATGTGTTCAG
TCCAGACCTTGATAATGTTGGAGAGGTAAAGTGTTGTGAGATAAATTTCGAAATTTTCAA
AGATCAATGACAAAGTTTTGATCTAATCTTATTCAACCTTTTTTTTTTGTGTGGTAGGGA
GTTTACAAGGGCCTGTACAAATTAACGCTAAAACAACTTAATAACACATTGCGAACGTTT
TTAAGGTACGGGGGGGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTT
CACCGCCGGGCCCTAAAAAATTTGCACCAAGCGGTCCACCCTGGTTTGCCCCACCAGGCA
AAAATCCGGTTTGATGGTGGTTCCAAA
   
Sequence Name 58-K113948-SALK_008144C-R210
GenBank Accession LN485693 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K113948-SALK_008144C-R210
GCCCTCGTAACTCCCTACCACACAAAAAAAAAAGGTTGAATAAAATTAAATCAAAACTTT
GTCATTGATCTTTGAAAATTTCAAAATTTATCTCACAACACTTTACCTCTCCAACATTAT
CAAGGTCTGGACTGAACACATGGAGTCTTGCACCCACCGGTTGTTTAATCAACAGCTTAA
ACCCTTCATCCAAACTCTTATTCTCAATCACTTCTCCTTTCAGGGGCACCCGGGGTATGT
GACAAGCAAATGGGGAACCCTTGAATGTACATCCCACATGTGCCCAATGTTCTCTTCTCG
GTAGCCCTTTCTTGAAAGCACATTCAAGGCATTCTTTGACAGTGAGTGGTTGCTCCCTCT
CCCTGCACATCTAACCTCTAATTCCAAACTTTTTCCACCAAATACGGATGCAAGTTT

Last Updated on 06.12.2018 16:11