Genotyping and primer details


Genotyping and primer details for SALK_008183c - At5g41570
Line ID SALK_008183c
Locus At5g41570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16625522 of line SALK_008183c Chr5:16625522
   
Insertion allele amplicon LBb3 - 44us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 44us: 5'-ATACATACTTTGGCCGGTTTAGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16626213
   
wt amplicon (over InsPos) 35vw - 44us
wt amplicon start Chr5:16625067
wt amplicon end Chr5:16626213
wt amplicon size 1147 bp
Locus specific primer 44us: 5'-ATACATACTTTGGCCGGTTTAGTG-3'
2nd locus specific primer 35vw: 5'-CACATCCGAGTATGAAATTGTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11