Sequences from confirmation amplicon


Confirmation sequences for line SALK_008183c and hit At5g41570 are shown.
Line ID SALK_008183c
Locus At5g41570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16625522
Primers used for PCR 44us - LBb3, show primer details
   
Sequence Name 36-K117945-SALK_008183C-44us
GenBank Accession LN485242 [GenBank]
Sequencing primer gene specific primer, 44us: 5'-ATACATACTTTGGCCGGTTTAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K117945-SALK_008183C-44us
ACAAATCGACAACGTATCATCTTTCAATGTCTTCCGGTTGATTATTTGTACAAGTTTTCT
CTCTCTCTGAAGCATGTTTTTCTTCTTTAGAATTCTCTAGTTACCAAATGTCTGGACCCT
AACTTCTCTCTGTTAAGTATGTAGAAAAATATCAAAGAAGTTGTAGAGAATGAGGGTGGG
TTTAATGAAGTTAGAGGAAACTACCTTTTCATACTTACATATGTAACTAAACTTGTTTTG
TCTGGTATTTGTCAAATCATCGGCGTAAACCGATCATAAACCAGAAATCAGGGGATAAAA
TTCAGAAACATTTGTAAAAAGTCGTAACTGTTACATTGGTTTAAAATCTGATCTACACAC
TTGTGACTTGTCACTTGGGAGATGTGGGTCGTCAAACGGTCAGTGGGGTGATTAACACCT
TAATCACCTTTAAGTAATTACACCATTAATTAAGCCATTATATATTCAAAGATTAGAGAA
ACTGGAGAGGAACTGGAGTTGCCTGAGAAGAGGATTAAGAGTCTCCATGAGCTTCTCACA
AGGATGGTTATGAACGCCTTCGTAGGTCGTTACGACGACGTTTGGATCTTTTGCCAATCT
CTGCACTTGTTTCTTCACGTTGCATGTGGTGTAAACAAATTGACGCTTAGACAACTTAAT
AACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGG
CAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCT
GGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGT
   
Sequence Name 01-K117944-SALK_008183C-R210
GenBank Accession LN484293 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K117944-SALK_008183C-R210
CATTTGTTACCCACATGCAACGTGAAGAAACAGTGCAGAGATTGGCAAAAGATCCAAACG
TCGTCGTAACGACCTACGAAGGCGTTCATAACCATCCTTGTGAGAAGCTCATGGAGACTC
TTAATCCTCTTCTCAGGCAACTCCAGTTCCTCTCCAGTTTCTCTAATCTTTGAATATATA
ATGGCTTAATTAATGGTGTAATTACTTAAAGGTGATTAAGGTGTTAATCACCCCACTGAC
CGTTTGACGACCCACATCTCCCAAGTGACAAGTCACAAGTGTGTAGATCAGATTTTAAAC
CAATGTAACAGTTACGACTTTTTACAAATGTTTCTGAATTTTATCCCCTGATTTCTGGTT
TATGATCGGTTTACGCCGATGATTTGACAAATACCAGACAAAACAAGTTTAGTTACATAT
GTAAGTATGAAAAGGTAGTTTCCTCTAACTTCATTAAACCCACCCTCATTCTCTACAACT
TCTTTGATATTTTTCTACATACTTAACAGAGAGAAGTTAGGGTCCAGACATTTGGTAACT
AGAGAATTCTAAAGAAGAAAAACATGCTTCAGAGAGAGAGAAAACTTGTACAAATAATCA
ACCGGAAGACATTTGAAAGATGATACGTTGTCGATTTTTGTTTGTGATTACTCTTTCAAA
TCCCAAAATTCATCACTAAACCGGCCAAAGTATGTA

Last Updated on 06.12.2018 16:11