Genotyping and primer details


Genotyping and primer details for SALK_008606c - At5g67300
Line ID SALK_008606c
Locus At5g67300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26854021 of line SALK_008606c Chr5:26854021
   
Insertion allele amplicon LBb3 - 51ew
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 51ew: 5'-CTCATGTAAAGCCCAGTAACAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26854533
   
wt amplicon (over InsPos) 28gc - 51ew
wt amplicon start Chr5:26853654
wt amplicon end Chr5:26854533
wt amplicon size 880 bp
Locus specific primer 51ew: 5'-CTCATGTAAAGCCCAGTAACAACA-3'
2nd locus specific primer 28gc: 5'-CAAATTGTGCGAATATTATCATGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11