Genotyping and primer details


Genotyping and primer details for SALK_008956c - At2g21300
Line ID SALK_008956c
Locus At2g21300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9117733 of line SALK_008956c Chr2:9117733
   
Insertion allele amplicon LBb3 - 12hd
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12hd: 5'-ACACCCAAAAGAAGATAAAGCAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9116808
   
wt amplicon (over InsPos) 02ix - 12hd
wt amplicon start Chr2:9116808
wt amplicon end Chr2:9117881
wt amplicon size 1074 bp
Locus specific primer 12hd: 5'-ACACCCAAAAGAAGATAAAGCAGA-3'
2nd locus specific primer 02ix: 5'-TTTGGTGACAGATAGAGTGTACCG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11