Sequences from confirmation amplicon


Confirmation sequences for line SALK_008956c and hit At2g21300 are shown.
Line ID SALK_008956c
Locus At2g21300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9117733
Primers used for PCR 12hd - LBb3, show primer details
   
Sequence Name 84-K112710-SALK_008956C-12hd
GenBank Accession LN486273 [GenBank]
Sequencing primer gene specific primer, 12hd: 5'-ACACCCAAAAGAAGATAAAGCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K112710-SALK_008956C-12hd
TTTTCCTTGCCTAGAACTCCGAGCAGAGCTTTCAACTGTCTACACAATGTTAACAATTTA
GATAAGGCACTGAGCAAAAAAAGAAAGGAAAATCTTTGTGCCTTAGATCCAGTGATAGAA
AAGAGCTTTACTAGTTTGATAATTTGATGAGATCTTGAACTTCTCTCATTTAGTGAAGTT
TCACCAATCTTCCGCTGTGCTGCAATAAGAAAATCAAGAAGTAAAGAAACATGATGAGAG
ATATTTCTAAGACAACATTTTATACAGAATAACCTGAGTTGCAAATCCTGAGCAAGCTCC
ATAACTATCAGCTCGTTGTAGTAACAGAAAATAGGTTGACTATTTGAGATTATTACCTTC
ACAAACTGAGATAAGTTCCTTCAAATGGTTCCAATCTCTCAGCGTTTCCTCTGTGGCTTT
TTCAACGGCGGCCCCTTTCTAAATGGAAGAAGAAGAAAAACACGAGAAGAGAGTCACACA
ACATTATTAACAAAAAGAACTCGCAGTAAGGAGAGAAAAAAAAATCTTTCACCTCAGGGT
CATCTCGTAGCCTCAGTGGTGTACTATCTGGGCTGAGAAGATCGCGGATGGCCTCATTGT
AGATCTCTATAGCCGAAAATTTCACAACAAATGCCCTATCTTCATGCTGCAGTGTCCAGT
CCAATTAGTGAAAAGAGGAATACATATCANAGTAGTATGCGAGAAAATCAGCCTTAATTT
TTGGCAGTACCTTAAATATATAATCAAATATGTCAGCCACAGCAAACTCAGTTATACCAG
ACATGGTGTATGTCTTTCCACTGCTCGTCTGACCATACGCGAAAATACTAGCTGGGAAAC
AATAAGTATTGAATCACTTGGCTAACTTAATAACAAACTGCAAGAGGATATATNGTGGTG
TAAACAAATTGACGCTNAGACNACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GGTGGTTTTTCTTTTTCACAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCNGGCC
CTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCC
   
Sequence Name 74-K112709-SALK_008956C-R210
GenBank Accession LN486105 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K112709-SALK_008956C-R210
TTTGTTACCCACAATATATCCTCTTGCAGTTTGTTATTAAGTTAGCCAAGTGATTCTATA
CTTATTGTTTCCCAGCTAGTATTTTCGCGTATGGTCAGACGAGCAGTGGAAAGACATACA
CCATGTCTGGTATAACTGAGTTTGCTGTGGCTGACATATTTGATTATATATTTAAGGTAC
TGCCAAAAATTAAGGCTGATTTTCTCGCATACTACTTTGATATGTATTCCTCTTTTCACT
AATTGGACTGGACACTGCAGCATGAAGATAGGGCATTTGTTGTGAAATTTTCGGCTATAG
AGATCTACAATGAGGCCATCCGCGATCTTCTCAGCCCAGATAGTACACCACTGAGGCTAC
GAGATGACCCTGAGGTGAAAGATTTTTTTTTCTCTCCTTACTGCGAGTTCTTTTTGTTAA
TAATGTTGTGTGACTCTCTTCTCGTGTTTTTCTTCTTCTTCCATTTAGAAAGGGGCCGCC
GTTGAAAAAGCCACAGAGGAAACGCTGAGAGATTGGAACCATTTGAAGGAACTTATCTCA
GTTTGTGAAGGTAATAATCTCAAATAGTCAACCTATTTTCTGTTACTACAACGAGCTGAT
AGTTATGGAGCTTGCTCAGGATTTGCAACTCAGGTTATTCTGTATAAAATGTTGTCTTAG
AAATATCTCTCATCATGTTTCTTTACTTCTTGATTTTCTTATTGCAGCACAGCGGAAGAT
TGGTGAAACTTCACTAAATGAGAGAAGTTCAAGATCTCATCAAATTATCAAACTAGTAAA
GCTCTTTTCTATCACTGGATCTAAGGCACAAAGATTTTCCTTTCTTTTTTTGCTCAGTGC
CTTATCTAAATTGTTAACATTGTGTAGACAGTTGAAAGCTCTGCTCGTGAGTTCTTAGGC
AAGGAAAACTCAACCACTCTCATGGCGAGTGTGGTATGTACTCATCTGCTTTATCTT

Last Updated on 06.12.2018 16:11