Genotyping and primer details


Genotyping and primer details for SALK_009348c - At3g51870
Line ID SALK_009348c
Locus At3g51870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19244910 of line SALK_009348c Chr3:19244910
   
Insertion allele amplicon LBb3 - 05tw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 05tw: 5'-GCAGACACATGGTATAAGACTTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19244447
   
wt amplicon (over InsPos) 15ue - 05tw
wt amplicon start Chr3:19244447
wt amplicon end Chr3:19245388
wt amplicon size 942 bp
Locus specific primer 05tw: 5'-GCAGACACATGGTATAAGACTTGG-3'
2nd locus specific primer 15ue: 5'-ATGGAGACTTATGATGGAACCAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11