Genotyping and primer details


Genotyping and primer details for SALK_009373c - At3g16910
Line ID SALK_009373c
Locus At3g16910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5774043 of line SALK_009373c Chr3:5774043
   
Insertion allele amplicon LBb3 - 48xh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 48xh: 5'-TTATGGAGGAGAAAGCTGGAAGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5774608
   
Insertion allele amplicon R204 - BN33
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BN33: 5'-GTGTGTTTTGCCAATGTTTGTTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5772918
   
wt amplicon (over InsPos) BN34 - BN33
wt amplicon start Chr3:5772918
wt amplicon end Chr3:5773965
wt amplicon size 1048 bp
Locus specific primer BN33: 5'-GTGTGTTTTGCCAATGTTTGTTAC-3'
2nd locus specific primer BN34: 5'-CCTTCTGTTCTCTTCTCCATGAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11