Genotyping and primer details


Genotyping and primer details for SALK_009578c - At3g10500
Line ID SALK_009578c
Locus At3g10500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3272401 of line SALK_009578c Chr3:3272401
   
Insertion allele amplicon LBb3 - 01cl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 01cl: 5'-TTGTTTCAACATTATTGCTCGACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3272786
   
Insertion allele amplicon LBb3 - 25cu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 25cu: 5'-CGAAAGATCTGCAATAAACCTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3271802
   
wt amplicon (over InsPos) 25cu - 01cl
wt amplicon start Chr3:3271802
wt amplicon end Chr3:3272786
wt amplicon size 985 bp
Locus specific primer 01cl: 5'-TTGTTTCAACATTATTGCTCGACT-3'
2nd locus specific primer 25cu: 5'-CGAAAGATCTGCAATAAACCTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11