Sequences from confirmation amplicon


Confirmation sequences for line SALK_009578c and hit At3g10500 are shown.
Line ID SALK_009578c
Locus At3g10500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3272401
Primers used for PCR 01cl - LBb3, show primer details
   
Sequence Name 75-K108073-SALK_009578C-R210
GenBank Accession LN486124 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K108073-SALK_009578C-R210
GTCATTTGTTTACCCCAATATATCCTGACCAGAAGATCTTGGAAGTGAAGATCATTTTTA
TGTCCATATGGATGACATTGATCAGGTTTGTGTTCTAGTATTTGATATCTCTTGATTAGC
TTTTGGTCGAATGTTGTGTTCCTTTGTATTCATTGAGGAATGAAAGAAGCTTGTGAAAAT
CTCTCCTTCTCATTCCCTGATTTGGTCAGAGTTCCATCTAGGCTGTTTATTATGTTCTTC
TAATTTTATGATTCGAATTTTATATCTTGCAGAAGTCTGAAAACTTTGTGGTCTATGATG
CCATTCCTATTCCGTTAAACTTTATTCACGGGGAGTCGAGCAATAATGTTGAAACA
Primers used for PCR 25cu - LBb3, show primer details
   
Sequence Name 85-K119306-SALK_009578C-25cu
GenBank Accession LN486291 [GenBank]
Sequencing primer gene specific primer, 25cu: 5'-CGAAAGATCTGCAATAAACCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K119306-SALK_009578C-25cu
ATCTGAGCCTTGGGATCTCCAGGTCAGTTCTTCAATATGTTGATGATTTGATCGATATTG
TATAGGAACCGATGATAGAATTTGAATTCTGTTTTGTATTTTGGTGGGAAATAGACAAGT
CGAGGCTGAAAAGTAGAGACTTGGAGTGGTACTTCTTCAGTATGCTTGATAAGAAGTATA
GAAATGGATCAAAGACGAATCGTGCGACTGAGATGGGATATTGGAAGACTACAGGGAAAG
ATCGAGAGATTCTTAATGGTTCAAAAGTCGTTGGGATGAAGAAGACTCTTGTTTATCACA
AAGGTCGAGCTCCTCGTGGCGAAAGAACCAATTGGGTTATGCATGAGTATCGGTTGGTTG
ATCAAGACCTTGATAAAACTGGTGTTCACCAAGTAAGACTTTGATCTTTTTGAGTTCCTT
GTTGTTTAATGGGATTTGTGTTGTTGACTTTGTTGTGGAATTGTGGGAAGGATGCGTTTG
TGCTTTGTAGGATTTTTCAGAAGAGTGGCTCCGGACCTAAAAATGGAGAGCAGTACGGTG
TAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCAAGTTTG
ATGGTGGTTC

Last Updated on 06.12.2018 16:11