Genotyping and primer details


Genotyping and primer details for SALK_009879c - At1g19220
Line ID SALK_009879c
Locus At1g19220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6631977 of line SALK_009879c Chr1:6631977
   
Insertion allele amplicon LBb3 - 45us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 45us: 5'-GTGATGATGATGAACAGGAGAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6632457
   
wt amplicon (over InsPos) 35vl - 45us
wt amplicon start Chr1:6631484
wt amplicon end Chr1:6632457
wt amplicon size 974 bp
Locus specific primer 45us: 5'-GTGATGATGATGAACAGGAGAGAA-3'
2nd locus specific primer 35vl: 5'-ACCTGTGGTAAGCAAGTGTCTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11