Genotyping and primer details


Genotyping and primer details for SALK_010185 - At1g21780
Line ID SALK_010185
Locus At1g21780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7654011 of line SALK_010185 Chr1:7654011
   
Insertion allele amplicon LBb3 - 50qz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 50qz: 5'-CCTCTCAGCTAGCTCAACTGTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7653419
   
wt amplicon (over InsPos) 08rz - 50qz
wt amplicon start Chr1:7653419
wt amplicon end Chr1:7654297
wt amplicon size 879 bp
Locus specific primer 50qz: 5'-CCTCTCAGCTAGCTCAACTGTTTT-3'
2nd locus specific primer 08rz: 5'-TGGATTCTTAGCTCAAACTTCGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11