Genotyping and primer details


Genotyping and primer details for SALK_010286c - At1g24180
Line ID SALK_010286c
Locus At1g24180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8562763 of line SALK_010286c Chr1:8562763
   
Insertion allele amplicon LBb3 - 52cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 52cs: 5'-ACCAACAGGAGCAGCTGTAATACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8562027
   
wt amplicon (over InsPos) 22dw - 52cs
wt amplicon start Chr1:8562027
wt amplicon end Chr1:8562944
wt amplicon size 918 bp
Locus specific primer 52cs: 5'-ACCAACAGGAGCAGCTGTAATACT-3'
2nd locus specific primer 22dw: 5'-TGTTCTCATGGTAAAGGAGGATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11