Sequences from confirmation amplicon


Confirmation sequences for line SALK_010286c and hit At1g24180 are shown.
Line ID SALK_010286c
Locus At1g24180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8562763
Primers used for PCR 52cs - LBb3, show primer details
   
Sequence Name 84-K109033-SALK_010286C-52cs
GenBank Accession LN486269 [GenBank]
Sequencing primer gene specific primer, 52cs: 5'-ACCAACAGGAGCAGCTGTAATACT-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K109033-SALK_010286C-52cs
GGCAACTTGCACGCCCGTTTCCAGCCAGTGCATCCATACCATNTCAATCAAAAACAAAGN
AAAAGATAATCATTCGTTAGCTTTAAGCTCTTAATCTCACCAATCAGAAAAACAACGGAT
ACAATTCTATTGGGTAAAGTAATAAAAAACTGAAGCAACTTCTGTAAAACAACAAAGATA
GAGACTTTGGAGACAATTCCTTTTGGTAGTTCTCACAAAGTTACACTTGATCCGAACACA
CAACTGTGAAGGTTTGTCCTTTCTCATTCAATTTGTGTCTAAAACCCTATCCTCACTACC
TAGCTGTTCCATAGTCGACCCAGTGGTCTAAAACACACAAGGAAACCCTGTATCAACTAA
AAGCGTGCTATAGACAACTCAGTCTCATCAAAGCAAAAGCATTGTAAAGCTCAAGATCAT
GCCAAAAGACAACTCAGTCTCACCTTCAAGCCAGGAACATAATCTCCACGCTTGAAATAA
GCCGGAGACTTAGCCGACCTCCATGTAGCCGTTCCCATTCCATCTAAACATCAAACAATC
CAAATCAACACAAAAGTCTCAACAATTCCACCAAACAAATTACAATTAACCAAAAAATAA
ACTCACAATGGTTATTCTCGCAAACAAGAATCGCAGGCAAATCCCACAAAGCCGAAATGT
TCAAGGCCTCAAACAACTTGACGCACCCGCACCAGCCTCATCCTTAT
   
Sequence Name 68-K109032-SALK_010286C-R210
GenBank Accession LN485948 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K109032-SALK_010286C-R210
GGATGAGGCTGGTGCGGGTGCGTCAAGTTGTTTGAGGCCTTGAACCCTTTAGATTTGTGG
GATTTGCCTGCGATTCTTGTTTGCGAGACTAACCATTGTGAGTTTATTTTTTGGTTAATT
GTAATTTGTTTGGTGGAATTGTTGAGACTTTTGTGTTGATTTGGATTGTTTGATGTTTAG
ATGGAATGGGAACGGCTACATGGAGGTCGGCTAAGTCTCCGGCTTATTTCAAGCGTGGAG
ATTATGTTCCTGGCTTGAAGGTGAGACTGAGTTGTCTTTTGGCATGATCTTGAGCTTTAC
AATGCTTTTGCTTTGATGAGACTGAGTTGTCTATAGCACGCTTTTAGTTGATACAGGGTT
TCCTTGTGTGTTTTAGACCACTGGGTCGACTATGGAACAGCTAGGTAGTGAGGATAGGGT
TTTAGACACAAATTGAATGAGAAAGGACAAACCTTCACAGTTGTGTGTTCGGATCAAGTG
TAACTTTGTGAGAACTACCAAAAGGAATTGTCTCCAAAGTCTCTATCTTTGTTGTTTTAC
AGAAGTTGCTTCAGTTTTTTATTACTTTACCCAATAGAATTGTATCCGTTGTTTTTCTGA
TTGGTGAGAGTGTGAACCTAAAGCTAACGAATGAATATCTTTT

Last Updated on 06.12.2018 16:11