Genotyping and primer details


Genotyping and primer details for SALK_010839c - At3g04120
Line ID SALK_010839c
Locus At3g04120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1082981 of line SALK_010839c Chr3:1082981
   
Insertion allele amplicon LBb3 - 10bb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 10bb: 5'-GATTTGGAATTGTTGAGGGTCTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1082444
   
wt amplicon (over InsPos) 02cx - 10bb
wt amplicon start Chr3:1082444
wt amplicon end Chr3:1083153
wt amplicon size 710 bp
Locus specific primer 10bb: 5'-GATTTGGAATTGTTGAGGGTCTTA-3'
2nd locus specific primer 02cx: 5'-CATTCGAGATCTGCTTCTTAGCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11