Genotyping and primer details


Genotyping and primer details for SALK_011187c - At4g28130
Line ID SALK_011187c
Locus At4g28130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13973759 of line SALK_011187c Chr4:13973759
   
Insertion allele amplicon LBb3 - 64sa
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 64sa: 5'-AGGTTTGCATTACCTTTGCTCTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13973176
   
wt amplicon (over InsPos) 13sx - 64sa
wt amplicon start Chr4:13973176
wt amplicon end Chr4:13974068
wt amplicon size 893 bp
Locus specific primer 64sa: 5'-AGGTTTGCATTACCTTTGCTCTTA-3'
2nd locus specific primer 13sx: 5'-AACACATATACACACACCACACGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11