Sequences from confirmation amplicon


Confirmation sequences for line SALK_011187c and hit At4g28130 are shown.
Line ID SALK_011187c
Locus At4g28130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13973759
Primers used for PCR 64sa - LBb3, show primer details
   
Sequence Name 39-K116392-SALK_011187C-64sa
GenBank Accession LN485282 [GenBank]
Sequencing primer gene specific primer, 64sa: 5'-AGGTTTGCATTACCTTTGCTCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K116392-SALK_011187C-64sa
GCTCTAAGTTTACATTAGTGGGACAGTTGTACAATATATTTCACGCTGTCTAAATTACAC
TAATATGTAATGTTTCCATTGATTTCATGACCTTCCAAAGCACATATCTAAAGCTTAGTT
GCACACAAGGATGGTTTTTTGCCTCTCTTTTTCACCCTTCTTCACAGTGAGTATATTTTT
ATTTTCTTATCCATTCTTTCCAATTTCGCTTGCCACTCATTAAAATTAGACGTCATCTTA
TAGTTTCGATGTAACTTGATTTCATGTGTATATAATTTTGCTCTCAATTGAATCTATGCC
GTTTGCTGCAAAAACATAGCTAAGCTTGCAAAAATTCAAATTTGTGATAGAAATGGCCAA
TGGAATGATCTTCATATTCCCCAAAGGTAACTGTATAAGTAAAAGGTTATGCAAGGCTTT
CTATTTGTACAATAGAGAATATAGTAATTAACATAAATTTTTGTTCTGGCAGCATAAGGT
CCATAGTATGTTTGAATCTTCCTAGTTTTTCTGGAGGACTAAATCCAGAAACAAATTGAC
AAATATATTGTCCAAATTGACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACG
TTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCC
CTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAG
GCGAAAATCCTGTTTGATGGTGGTTCCA
   
Sequence Name 14-K116391-SALK_011187C-R210
GenBank Accession LN484676 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K116391-SALK_011187C-R210
TCAATTTGTTTCTGGATTTAGTCCTCCAGAAAAACTAGGAAGATTCAAACATACTATGGA
CCTTATGCTGCCAGAACAAAAATTTATGTTAATTACTATATTCTCTATTGTACAAATAGA
AAGCCTTGCATAACCTTTTACTTATACAGTTACCTTTGGGGAATATGAAGATCATTCCAT
TGGCCATTTCTATCACAAATCTGAATTTTTGCAAGCTTAGCTATGTTTCTGCAGCAAACG
GCATAGATTCAATTGAGAGCAAAATTATATACACATGAAATCAAGTTACATCGAAACTAT
AAGATGACGTCTAATTTTAATGAGTGGCAAGCGAAATTGGAAAGAATGGATAAGAAAATA
AAAATATACTCACTGTGAAGAAGGGTGAAAAAGAGAGGCAAAAAACCATCCTTGTGTGCA
ACTAAGCTTTAGATATGTGCTCTGGAAGGTCATGAAATCAATGGAAACATTACATATTAG
TGTAATTTAGTCACGTGAAATATATTGTACAACTGTCCCACTAATGTAAACATTAGAGCC
ACATCAATTGAAGACAACATAAAAAAAAAACTTTAAGAGCAAA

Last Updated on 06.12.2018 16:11