Genotyping and primer details


Genotyping and primer details for SALK_011390 - At2g16250
Line ID SALK_011390
Locus At2g16250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7042166 of line SALK_011390 Chr2:7042166
   
Insertion allele amplicon R204 - 01as
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 01as: 5'-ATGCATAGAACCAGAATAATTGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7041513
   
wt amplicon (over InsPos) 04bs - 01as
wt amplicon start Chr2:7041513
wt amplicon end Chr2:7042272
wt amplicon size 760 bp
Locus specific primer 01as: 5'-ATGCATAGAACCAGAATAATTGGC-3'
2nd locus specific primer 04bs: 5'-GAGCGTTATCAAAGCTTATCCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11