Genotyping and primer details


Genotyping and primer details for SALK_012128c - At3g23070
Line ID SALK_012128c
Locus At3g23070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8203416 of line SALK_012128c Chr3:8203416
   
Insertion allele amplicon R212 - 18cr
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer 18cr: 5'-GTGGAGATATGGTTAAGGGATGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8202922
   
wt amplicon (over InsPos) 01ci - 18cr
wt amplicon start Chr3:8202922
wt amplicon end Chr3:8203838
wt amplicon size 917 bp
Locus specific primer 18cr: 5'-GTGGAGATATGGTTAAGGGATGAG-3'
2nd locus specific primer 01ci: 5'-TCTCCCTTCTTTTCGATAATTCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11