Genotyping and primer details


Genotyping and primer details for SALK_012134c - At1g07750
Line ID SALK_012134c
Locus At1g07750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2404967 of line SALK_012134c Chr1:2404967
   
Insertion allele amplicon LBb3 - 70by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 70by: 5'-AACCTTGGAACAATGAAGAGAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2404608
   
wt amplicon (over InsPos) 15da - 70by
wt amplicon start Chr1:2404608
wt amplicon end Chr1:2405356
wt amplicon size 749 bp
Locus specific primer 70by: 5'-AACCTTGGAACAATGAAGAGAGAA-3'
2nd locus specific primer 15da: 5'-TGCTTAATCACGTCTTAATCGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11