Genotyping and primer details


Genotyping and primer details for SALK_012144c - At1g02980
Line ID SALK_012144c
Locus At1g02980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:680649 of line SALK_012144c Chr1:680649
   
Insertion allele amplicon LBb3 - 71by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 71by: 5'-TACCTTGAACATTTTGCTGAGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:681028
   
wt amplicon (over InsPos) 02dg - 71by
wt amplicon start Chr1:680250
wt amplicon end Chr1:681028
wt amplicon size 779 bp
Locus specific primer 71by: 5'-TACCTTGAACATTTTGCTGAGATG-3'
2nd locus specific primer 02dg: 5'-CTACCCATTGAAATGGTAAGTCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11