Genotyping and primer details


Genotyping and primer details for SALK_012199 - At4g28360
Line ID SALK_012199
Locus At4g28360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14029963 of line SALK_012199 Chr4:14029963
   
Insertion allele amplicon LBb3 - 07rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 07rb: 5'-ATTGATATAATCCCGCTTCTTCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14029505
   
wt amplicon (over InsPos) 25rz - 07rb
wt amplicon start Chr4:14029505
wt amplicon end Chr4:14030568
wt amplicon size 1064 bp
Locus specific primer 07rb: 5'-ATTGATATAATCCCGCTTCTTCCT-3'
2nd locus specific primer 25rz: 5'-CTGAGATTGGAGTTTCTTGTTTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11