Genotyping and primer details


Genotyping and primer details for SALK_012298 - At1g13800
Line ID SALK_012298
Locus At1g13800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4732293 of line SALK_012298 Chr1:4732293
   
Insertion allele amplicon R204 - AW41
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AW41: 5'-GGTCGTTTAAATGGCAATATCTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4731568
   
Insertion allele amplicon LBb3 - AW42
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer AW42: 5'-CTCAATCCTTCAATGCTATTGTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4732613
   
wt amplicon (over InsPos) AW42 - AW41
wt amplicon start Chr1:4731568
wt amplicon end Chr1:4732613
wt amplicon size 1046 bp
Locus specific primer AW41: 5'-GGTCGTTTAAATGGCAATATCTGT-3'
2nd locus specific primer AW42: 5'-CTCAATCCTTCAATGCTATTGTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11