Genotyping and primer details


Genotyping and primer details for SALK_012424c - At2g20430
Line ID SALK_012424c
Locus At2g20430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8809078 of line SALK_012424c Chr2:8809078
   
Insertion allele amplicon LBb3 - 29vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29vi: 5'-GAAGAAATACAAATCGGAAATCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8809450
   
wt amplicon (over InsPos) 57vw - 29vi
wt amplicon start Chr2:8808588
wt amplicon end Chr2:8809450
wt amplicon size 863 bp
Locus specific primer 29vi: 5'-GAAGAAATACAAATCGGAAATCCA-3'
2nd locus specific primer 57vw: 5'-AACAATTGGTGTGTTGTGTTCAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11