Genotyping and primer details


Genotyping and primer details for SALK_012425 - At5g63570
Line ID SALK_012425
Locus At5g63570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25453544 of line SALK_012425 Chr5:25453544
   
Insertion allele amplicon LBb3 - 38oh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 38oh: 5'-TTGTTAAAGCTGGTAGTGGTGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25452705
   
wt amplicon (over InsPos) 09px - 38oh
wt amplicon start Chr5:25452705
wt amplicon end Chr5:25453579
wt amplicon size 875 bp
Locus specific primer 38oh: 5'-TTGTTAAAGCTGGTAGTGGTGTTG-3'
2nd locus specific primer 09px: 5'-ACTGTATATCCTCGGGAGTGTGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11