Sequences from confirmation amplicon


Confirmation sequences for line SALK_012425 and hit At5g63570 are shown.
Line ID SALK_012425
Locus At5g63570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25453544
Primers used for PCR 38oh - LBb3, show primer details
   
Sequence Name 19-K115143-SALK_012425C-38oh
GenBank Accession LN484823 [GenBank]
Sequencing primer gene specific primer, 38oh: 5'-TTGTTAAAGCTGGTAGTGGTGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115143-SALK_012425C-38oh
ATACTTTAACAGCTCCGTACAATGATCTTGAAGCTGTTGAGAAGCTCTTTGCTGCGCATA
AAGGGGAGATTTCTGCGGTAATTCTCGAACCTGTTGTTGGTAACTCCGGATTCATCCCTC
CTACTCCGGAATTCATCAACGGTTTACGCCAGCTTACTAAAGACAATGGTGTTCTTCTCA
TCTTCGATGAAGTCATGACTGGATTTCGTCTAGCCTATGGTGGAGCCCAAGAATACTTTG
GAATCACGCCTGATCTAACTACTCTTGGTAAAATTATTGGTGGGGGTCTTCCCGTGGGAG
CATACGGTGGTAGACGTGATATTATGGAGATGGTAAGATCTGGCTCTAGACCTTTAACAA
GCTTCAAAACTAGGGGTGGCCATGTACCAATGTTGCTATTCTTTTTTTTTTTTCGAGGGA
GCCCCCG
   
Sequence Name 03-K115142-SALK_012425C-R210
GenBank Accession LN484348 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115142-SALK_012425C-R210
GTCATTTGTTTCCCACAATTTGTCTAACCCGGCCTCGAACTGAGACGGTGCAAAGTAAAC
ACCTTCTTCCAACATTCCCCTGAAAAACCTACCAAACTTCTCTGTGTCGCTCTTCTTTGA
GTCTGCAAAGTTGTAAACCGGTCCTTCTGCGAAAAAGAATCCAAACATACCGCTTATGTA
ACCCCCGCACATTGGATGCCCGGTTTTCTTTCCGGCTTCTAAGATTCCGTTGGTTAGTTC
CTTCGTGATCTTGTCTAAATACTCATATGTTCCTGCCTGCTTTAGCCGCTTCAGCGTGTG
AATCCCTGCAGTCATTGCCAGCGGATTACCACTTAGAGTCCCGGCTTGGTACATTGGTCC
AGCGGGAGCAACCTACAAAAAAAAAAAAAAAAACCCACCTGGGGACCGGGCCCCCCCTAT
TTTTAAAACTTGTTAAAGGGTTAAAACCAAATTTTACCATTCCCAAAAAATCACGTCTAA
CACCGTAGGCCCCCCGGGGAAAACCCCCCCCAATTATTTTCCCAAAAATATTTAAAATGG
GGGGGATTCCAAAATTTTTTGGGGCCCCCCCAAAGGGAAAAAAAAACCCATTCTGAAATT
TCCCAAAAAAAAAAAAAACCCCCTTTTTTTTTAGAACCTGGCGTAAACCGTTAAAAAATT
CCGAAAAAAGAGGGAAAAATCCGGATTTTCCAACAAAGGGTTTAAAAATTCCCCCAAAAT
TTCCCCCTTTATGCCCCGCAAAAAATTTTTAAAAAGTTTCAAAA

Last Updated on 06.12.2018 16:11