Genotyping and primer details


Genotyping and primer details for SALK_012558c - At5g66140
Line ID SALK_012558c
Locus At5g66140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26437660 of line SALK_012558c Chr5:26437660
   
Insertion allele amplicon LBb3 - 22mh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22mh: 5'-ATTAACAAAGCAAGGATCGAGTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26438040
   
wt amplicon (over InsPos) 09oj - 22mh
wt amplicon start Chr5:26437231
wt amplicon end Chr5:26438040
wt amplicon size 810 bp
Locus specific primer 22mh: 5'-ATTAACAAAGCAAGGATCGAGTGT-3'
2nd locus specific primer 09oj: 5'-ATGAGCAACAGCAAATTTATCCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11