Sequences from confirmation amplicon


Confirmation sequences for line SALK_012558c and hit At5g66140 are shown.
Line ID SALK_012558c
Locus At5g66140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26437660
Primers used for PCR 22mh - LBb3, show primer details
   
Sequence Name 26-K114624-SALK_012558C-22mh
GenBank Accession LN485036 [GenBank]
Sequencing primer gene specific primer, 22mh: 5'-ATTAACAAAGCAAGGATCGAGTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K114624-SALK_012558C-22mh
ACATCACTCGCTACATTGCTGGCCTTCACAGAAGTATACCCAAAGTGGTGGTGTCAGACC
CTTCGGTCTTTCTACTCTTATCGTTGGCTTTGACCCTTACTCTCGCCTCCCTTCCCTATA
TCAGACTGATCCTTCTGGGACTTTCTCTGCTTGGAAAGCTAATGCTACCGGCAGAAACTC
CAACTCTATTAGGGAATTCCTCGAGAAGAACTACAAAGAATCCTCTGGCCAAGAAACTAT
TAAACTCGCTATCCGTGCTCTGCTTGAGGTTGGTGCCCTCTCCGTCCTAGCTGATATTTT
GTTATTTCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACAT
TGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGC
TGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGC
CCCAGCAGGCGAAAATCCTGTTTGATGGAGGTTCCAA
   
Sequence Name 10-K114623-SALK_012558C-R210
GenBank Accession LN484558 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K114623-SALK_012558C-R210
TATATCCTGAAATACAAAATATCAGCTAGGACGGAGAGGGCACCAACCTCAAGCAGAGCA
CGGATAGCGAGTTTAATAGTTTCTTGGCCAGAGGATTCTTTGTAGTTCTTCTCGAGGAAT
TCCCTAATAGAGTTGGAGTTTCTGCCGGTAGCATTAGCTTTCCAAGCAGAGAAAGTCCCA
GAAGGATCAGTCTGATATAGGGAAGGGAGGCGAGAGTAAGGGTCAAAGCCAACGATAAGA
GTAGAAAGACCGAAGGGTCTGACACCACCACTTTGGGTATACTTCTGTTGAAGGCCAGCA
ATGTAGCGAGTGATGTACTCAACAGTGACAGGGTCCTCAAGTGTAAGCCTGTGGCTTTGA
CACTCGATCCTTGTTTTGTTAA

Last Updated on 06.12.2018 16:11