Genotyping and primer details


Genotyping and primer details for SALK_012841c - At4g17980
Line ID SALK_012841c
Locus At4g17980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9979080 of line SALK_012841c Chr4:9979080
   
Insertion allele amplicon LBb3 - 30dh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 30dh: 5'-GCAGTCAATTGCTCTATCACTGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9978144
   
wt amplicon (over InsPos) 85dy - 30dh
wt amplicon start Chr4:9978144
wt amplicon end Chr4:9979213
wt amplicon size 1070 bp
Locus specific primer 30dh: 5'-GCAGTCAATTGCTCTATCACTGAG-3'
2nd locus specific primer 85dy: 5'-CATTTGGATTTCTCCTGATTTCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11