Genotyping and primer details


Genotyping and primer details for SALK_012997c - At1g62300
Line ID SALK_012997c
Locus At1g62300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23018227 of line SALK_012997c Chr1:23018227
   
Insertion allele amplicon R212 - 68wt
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer 68wt: 5'-GTTTCCCTCGTAGGTTGTAATCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23017451
   
Insertion allele amplicon LBb3 - AW93
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer AW93: 5'-GATTTCGTGTGAAGAAGGAAGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23018836
   
wt amplicon (over InsPos) AW94 - AW93
wt amplicon start Chr1:23017927
wt amplicon end Chr1:23018836
wt amplicon size 910 bp
Locus specific primer AW93: 5'-GATTTCGTGTGAAGAAGGAAGAAC-3'
2nd locus specific primer AW94: 5'-AATTCACCTTCTGAATTTTGTTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11