Genotyping and primer details


Genotyping and primer details for SALK_013055c - At1g05900
Line ID SALK_013055c
Locus At1g05900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1788285 of line SALK_013055c Chr1:1788285
   
Insertion allele amplicon LBb3 - 23mh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23mh: 5'-GATCAAAACAGCAGGTTTTCTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1787675
   
wt amplicon (over InsPos) 06ng - 23mh
wt amplicon start Chr1:1787675
wt amplicon end Chr1:1788832
wt amplicon size 1158 bp
Locus specific primer 23mh: 5'-GATCAAAACAGCAGGTTTTCTTTC-3'
2nd locus specific primer 06ng: 5'-GATGAGCAATCTTAGGACCAACTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11