Genotyping and primer details


Genotyping and primer details for SALK_013130 - At5g27700
Line ID SALK_013130
Locus At5g27700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9807551 of line SALK_013130 Chr5:9807551
   
Insertion allele amplicon LBb3 - CF90
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CF90: 5'-CTCTCGCTTTCTTCATCATCTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9808502
   
wt amplicon (over InsPos) CF91 - CF90
wt amplicon start Chr5:9807323
wt amplicon end Chr5:9808502
wt amplicon size 1180 bp
Locus specific primer CF90: 5'-CTCTCGCTTTCTTCATCATCTCTT-3'
2nd locus specific primer CF91: 5'-CATTCCGATTCGAACTCTAAAGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11