Sequences from confirmation amplicon


Confirmation sequences for line SALK_013130 and hit At5g27700 are shown.
Line ID SALK_013130
Locus At5g27700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:9807551
Primers used for PCR CF90 - LBb3, show primer details
   
Sequence Name 54-K107479-SALK_013130C-CF90
GenBank Accession LN485598 [GenBank]
Sequencing primer gene specific primer, CF90: 5'-CTCTCGCTTTCTTCATCATCTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K107479-SALK_013130C-CF90
TCTCTCGCTCTCTACTCAGGTAGGATAATCGTACTTTTACTTAATCTTCTCTTCGTCTCG
ATTTATCAGAAATATATGGACTTTTGCCAATATAGCCGATGGTTTAGGGTAGAAGAACCA
TGATGGATAAATAGTATTGAATCTGTAGTTTTTGTAACCCTAGATCTTCAAATTCGTATT
ATTGTTCTGTTAGATCTGTTTGTTACTCAACGAATTCGATTATTCCTTTGTTGTCTTGAA
TCCTTCTCTGATGTTACTTTCACTTACAGTCATTTTCGATCTCAGTGTATGTCACTGTTG
TTGGTTCTTGTCTAATATAAGATAGTTCTGTTTTTTCTTTCTTTTTGCATTGTTTGGATA
TATAGGACTCGTTTACTCTTTCAAAGCTAAGCTTTCAAGATGCAAAACGAAGAGGGTCAG
GTCACTGAGCTTTACATTCCTAGGAAATGGTAATGAGAATCATTTCTTCTTCTTTTGTTT
TGGTCTCTGTTTTGTGTTCTCTCATTAACTAAGAGAAGATTGGTTCTTGTTTGTTTGTTT
TGACTGAATATTGGCAGTTCTGCTACTAACCGGTTGATCACATCCAAGGATCATGCCTCT
GTTCAGCTCAACATTGGTCATTTAGATGCTAATGGCTTGTACACCGGACAGTTCACAACC
TTTGCTCTCTGCGGTTTTGTCCGTGCTCAGGTTTGTAACAATTTCTCAATGCTTTGTCCA
TAATTGAGTCAGAAGAGAGTTGATTTGTTTTGTGTGTATCATAAGAGAGTTGAACAACCA
ACCCTTTGAATTTCTACTTATCTTCATAGGTTAATTAACTATTGATGTACACGATTGCAG
GGAGACGCTGACAGTGGTGTCGACAGGTTGTGGCAGAAGAAGAAGGTTGAAGCCAACTTA
ATAACACATTGCGGACGTTTTTTATGTACTTGGGGTGGTTTTTCTTT
   
Sequence Name 70-K107480-SALK_013130C-LBb3
GenBank Accession LN485998 [GenBank]
Sequencing primer T-DNA primer, LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K107480-SALK_013130C-LBb3
GGGCAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATC
AGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCCAGTACATTAAAAACGTCCGC
AATGTGTTATTAAGTTGGCTTCAACCTTCTTCTTCTGCCACAACCTGTCGACACCACTGT
CAGCGTCTCCCTGCAATCGTGTACATCAATAGTTAATTAACCTATGAAGATAAGTAGAAA
TTCAAAGGGTTGGTTGTTCAACTCTCTTATGATACACACAAAACAAATCAACTCTCTTCT
GACTCAATTATGGACAAAGCATTGAGAAATTGTTACAAACCTGAGCACGGACAAAACCGC
AGAGAGCAAAGGTTGTGAACTGTCCGGTGTACAAGCCATTAGCATCTAAATGACCAATGT
TGAGCTGAACAGAGGCATGATCCTTGGATGTGATCAACCGGTTAGTAGCAGAACTGCCAA
TATTCAGTCAAAACAAACAAACAAGAACCAATCTTCTCTTAGTTAATGAGAGAACACAAA
ACAGAGACCAAAACAAAAGAAGAAGAAATGATTCTCATTACCATTTCCTAGGAATGTAAA
GCTCAGTGACCTGACCCTCTTCGTTTTGCATCTTGAAAGCTTAGCTTTGAAAGAGTAAAC
GAGTCCTATATATCCAAACAATGCAAAAAGAAAGAAAAAACAGAACTATCTTATATTAGA
CAAGAACCAACAACAGTGACATACACTGAGATCGAAAATGACTGTAAGTGAAAGTAACAT
CAGAGAAGGATTCAAGACAACAAAGGAATAATCGAATTCGTTGAGTAACAAACAGATCTA
ACAGAACAATAATACGAATTTGAAGATCTAGGGTTACAAAAACTACAGATTCAATACTAT
TTATCCATCATGGGTCTTCTACCCTAACCATCGGCTATATTGGCAAAAGTCCATATATTT
CTGATAAA

Last Updated on 06.12.2018 16:11